Coding

Part:BBa_K3038003:Design

Designed by: Anais CANTEAU   Group: iGEM19_Poitiers   (2019-10-15)
Revision as of 12:39, 16 October 2019 by Bvannier (Talk | contribs) (→‎Design Notes)


FadM C-ter Flag-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been designed to meet the RFC10 standard.

GC content has been optimized.

a Flag Tag has been added in the C term part of the sequence.

Source

Sequence synthesis by IDT

References

Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80. doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610