Primer

Part:BBa_K2982008:Design

Designed by: Tsang Hoi Yeung, Ocean   Group: iGEM19_HK_GTC   (2019-10-16)
Revision as of 12:29, 16 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Reverse primer for amplifying BBa_E0040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It have a XhoI restriction site that allow the cutting of part. It also contain linkage sequence of PET-28a vector between the RE site and the GFP gene.


Source

iGEM registry

References