Coding
CMYC 6HIS

Part:BBa_K3038000:Design

Designed by: Marine LAVAUD   Group: iGEM19_Poitiers   (2019-10-08)
Revision as of 12:23, 16 October 2019 by Bvannier (Talk | contribs)


Alcohol dehydrogenase - Geobacillus stearothermophilus cMYC and 6His N-terminal tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

C MYC and  6His Tags have been added on the Nterminal part of the sequence.

The sequence is flanked with the sequence of EcoRI and XbaI on the 5'end and the SpeI and PstI on the 3' end.

The sequence is cloned in pSB1A3 . This design was necessary because the construction will be inserted later on in a chloramphenicol and kanamycin resistant strain.

The RFC10 standard was followed.

THe GC content has been optimized for the synthesis by IDT

Source

AUTHORS   Robinson,G.A., Bailey,C.J. and Dowds,B.C.

TITLE     Gene structure and amino acid sequences of alcohol dehydrogenases

          of Bacillus stearothermophilus

JOURNAL   Biochim. Biophys. Acta 1218 (3), 432-434 (1994)

References

Gene structure and amino acid sequences of alcohol dehydrogenases of Bacillus stearothermophilus. Robinson,G.A., Bailey,C.J. and Dowds,B.C. JOURNAL Biochim. Biophys. Acta 1218 (3), 432-434 (1994)

Overexpression, Purification, and Biochemical Characterization of the Thermostable NAD-dependent Alcohol Dehydrogenase from Bacillus stearothermophilus. SHIM, EUN-JUNG, SANG-HOON JEON, AND KWANG-HOON KONG. J. Microbiol. Biotechnol. (2003), 13(5), 738–744