Signalling
Part:BBa_K3225015
Designed by: Yaran Zhao Group: iGEM19_iBowu-China (2019-10-16)
J23101-LuxR-pLux-LacZ
This device is designed for detect quorum sensing molecule(AHL)of Erwinia Carotovora in cell-free system. It consists of LuxR(quorum sensing transcriptional factor, BBa_C0062) drived by a constitutive promoter J23101 and LacZ(BBa_I732017) as a repoter drived by the binding promoter of LuxR(pLux, BBa_R0062).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//cds/reporter
efficiency |