Signalling

Part:BBa_K3225014

Designed by: Yaran Zhao   Group: iGEM19_iBowu-China   (2019-10-16)
Revision as of 11:46, 16 October 2019 by Registry (Talk | contribs)

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J23101-LuxR-pLux-GFP

This device is designed for detect quorum sensing molecule（AHL）of Erwinia Carotovora in cell-free system. It consists of LuxR(quorum sensing transcriptional factor, BBa_C0062) drived by a constitutive promoter J23101 and GFP as a repoter drived by the binding promoter of LuxR(pLux, BBa_R0062).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1037


[edit]
Categories
//cds/transcriptionalregulator/activator
Parameters
efficiency