Coding

Part:BBa_K3096007

Designed by: Famke Baeuerle, Lina Widerspick, Patrick Mueller, Jakob Keck   Group: iGEM19_Tuebingen   (2019-10-14)
Revision as of 11:40, 16 October 2019 by Famosa (Talk | contribs) (→‎References)

Type I CRISPR/Cas system surveillance complex Cascade ABCDE

This is a part consisting of all cofactors necessary for the functionality of the CRISPR/Cas3 system. It works if utilised with BBa_K3096001 and the respective CRISPR arrays targeting the to-be-degraded sequences.

Usage & Biology

In the type I CISPR/Cas system the crRNA guides the surveillance complex Cascade ABCDE to complementary DNA sequence. The DNA is unwound, generating the so-called R-loop. The Cas3 helicase-nuclease protein attaches to this R-loop and unidirectionally degrades the DNA. Hereby, the large Cas3/Cascade complex pulls the substrate DNA towards itself, introducing single strand breaks.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Xia, Peng-Fei, et al. "Synthetic genetic circuits for programmable biological functionalities." Biotechnology advances (2019).
  2. Terns, Michael P., and Rebecca M. Terns. "CRISPR-based adaptive immune systems." Current opinion in microbiology 14.3 (2011): 321-327.
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Parameters
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