Composite

Part:BBa_K3096051

Designed by: Jakob Keck, Patrick Mueller, Lina Widerspick, Famke Baeuerle   Group: iGEM19_Tuebingen   (2019-10-15)
Revision as of 11:40, 16 October 2019 by Famosa (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

CRISPR array that targets the genomic DNA of E.coli - expression controlled via the Mnt repressor

The CRISPR array sequence needed to guide the Cas3 nuclease to its cut sites in the genome of E.coli under the control of the Mnt promoter. When Mnt is present the array is not transcribed and the CRISPR/Cas3 System lacks ist gRNA and is inactive. Combined with the GlcNAc-6-P responsive repression system (BBa_K3096048) the expression of the array can be controlled.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Xia, Peng-Fei, et al. "Synthetic genetic circuits for programmable biological functionalities." Biotechnology advances (2019).
  2. Terns, Michael P., and Rebecca M. Terns. "CRISPR-based adaptive immune systems." Current opinion in microbiology 14.3 (2011): 321-327.
[edit]
Categories
Parameters
None