Coding

Part:BBa_K3002037:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-16)
Revision as of 11:32, 16 October 2019 by Kleind (Talk | contribs)


Wildtype MHETase for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 765
    Illegal PstI site found at 1089
    Illegal PstI site found at 1432
    Illegal PstI site found at 2242
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 765
    Illegal PstI site found at 1089
    Illegal PstI site found at 1432
    Illegal PstI site found at 2242
    Illegal NotI site found at 1100
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 765
    Illegal PstI site found at 1089
    Illegal PstI site found at 1432
    Illegal PstI site found at 2242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 765
    Illegal PstI site found at 1089
    Illegal PstI site found at 1432
    Illegal PstI site found at 2242
    Illegal NgoMIV site found at 698
    Illegal NgoMIV site found at 1159
    Illegal NgoMIV site found at 1189
    Illegal NgoMIV site found at 1504
    Illegal NgoMIV site found at 1522
    Illegal NgoMIV site found at 1558
    Illegal NgoMIV site found at 2201
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is a fusion of the parts BBa_K3002029 and BBa_K3002005, which were created in order to synthesize the codon-optimized MHETase gene.


Source

This part originates from Ideonella sakaiensis (<a href="https://science.sciencemag.org/content/351/6278/1196.full">Yoshida, S., 2016</a>) and is condon-optimized for Chlamydomonas reinhardtii.

References