Coding

Part:BBa_K2984033:Design

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 11:06, 16 October 2019 by Registry (Talk | contribs)

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L1c-AR-ARS-YFP-Rbcs2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 953
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 268
    Illegal PstI site found at 953
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 953
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 953
    Illegal NgoMIV site found at 1430
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Secretion vector for C.reinhardtii designed for Transformation in Clamydomnasd and further cloning into Level 2 backbone with MoClo Standard


Source

The construct consist of the four different Level 0 parts AR (BBa_K2984025),ARS (BBa_K2984000), YFP (BBa_K2984017) and Rbcs2 (BBa_K2984018). which were cloned into the level 1c Backbone (BBa_K2984002) using the MoClo Standard.

References