Coding
Part:BBa_K3102012
Designed by: Sarah DUQUESNE Group: iGEM19_Ionis_Paris (2019-10-03)
Revision as of 10:53, 16 October 2019 by ChloeDOIZELET (Talk | contribs)
TEV Protease
TEV protease is the 27 kDa catalytic domain of the Nuclear Inclusion is a (NIa) protein encoded by the tobacco etch virus (TEV). Its sequence specificity is far more stringent than the sequence of factor Xa, thrombin, or enterokinase. TEV protease is a very useful reagent for cleaving fusion proteins.
Consequently, it’s an highly specific cysteine protease that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)(BBa_K3102041) and cleaves between the Gln and Gly/Ser residues.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 492
Illegal NgoMIV site found at 798
Illegal AgeI site found at 153 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |