Composite

Part:BBa_K3102032:Design

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 10:48, 16 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎Design Notes)

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T7-RBS-ZWF-Ter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 810
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 810
    Illegal NotI site found at 691
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1367
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 810
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 810
    Illegal NgoMIV site found at 83
    Illegal NgoMIV site found at 800
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated the ZWF sequence with a Thymine into Cysteine at the 799bp position (T799C) because of the PstI enzyme (BBa_K3102008)

Source

Corynebacterium glutamicum, sequence found through Ebi https://www.ebi.ac.uk/ena/data/view/BAB98969

Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Zhao J. et al., "Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate." Metab. Eng. 6:164-174(2004)