Coding

Part:BBa_K3102008:Design

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-03)
Revision as of 10:45, 16 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


ZWF (Glucose-6-phosphate 1-dehydrogenase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 775
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 775
    Illegal NotI site found at 656
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 775
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 775
    Illegal NgoMIV site found at 48
    Illegal NgoMIV site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated a Thymine into Cysteine at the 799bp position (T799C) because of the PstI enzyme

Source

Corynebacterium glutamicum, sequence found through Ebi https://www.ebi.ac.uk/ena/data/view/BAB98969

References

Zhao J. et al., "Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate." Metab. Eng. 6:164-174(2004)