Composite

Part:BBa_K3202031

Designed by: Heyuan Ni   Group: iGEM19_BHSF_ND   (2019-10-16)
Revision as of 10:36, 16 October 2019 by Registry (Talk | contribs)

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Xyls-Pc-Pm-sfGFP

This composite part is designed as a genetic circuit that is used as a negative control group of out later experiment. In our study, we aimed to produce a system in which the coding sequence in the middle of the bistable system can be completely concealed. That is, there is no leakage during the normal phase of the functioning genetic circuit. Therefore, this composite part that encodes the Xyls-pC-pM-sfGFP is used as a reference of what the bacterial colony should be like when there exists leakage, which means our design of the "lock" system failed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1126
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 993
    Illegal NheI site found at 1016
    Illegal SpeI site found at 1126
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1126
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1126
    Illegal NgoMIV site found at 754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 217
    Illegal SapI.rc site found at 1165


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Parameters
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