Coding
TesA Cterm

Part:BBa_K3038002

Designed by: Anne-Claire BOISSON   Group: iGEM19_Poitiers   (2019-10-10)
Revision as of 10:28, 16 October 2019 by Bvannier (Talk | contribs)

TesA : GenBank: EG11542

Cloning of Acyl ACP thioesterase gene for 2-nonanone production

In order to produce the molecule of interest 2-nonanone, we worked with the Lawrence Berkeley National Laboratory, USA which is working on biofuels and modified E. coli strain and obtain a production of 2-nonanone. This production is possible using free fatty acids as substrate.

Here we present the cloning of thioesterase I (TesA), an enzyme involved in the synthesis of free fatty acids in E. coli.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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