Composite

Part:BBa_K2969043

Designed by: Liangchen Zhuo   Group: iGEM19_UCAS-China   (2019-10-16)
Revision as of 10:21, 16 October 2019 by Registry (Talk | contribs)

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J23119-TlpA36-pTlpA-mRFP

This part contains the coding gene of the thermo-sensitive transcription factor TlpA36 under the constitutive promoter J23119 and a reporter gene mRFP under the promoter of TlpA36 pTlpA. When the temperature is below the transition temperature of TlpA36, TlpA is active to repress the transcription of mRFP so that there will be little fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 589
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1177
    Illegal AgeI site found at 1926
    Illegal AgeI site found at 2038
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None