Composite

Part:BBa_K2969042

Designed by: Liangchen Zhuo   Group: iGEM19_UCAS-China   (2019-10-16)
Revision as of 10:18, 16 October 2019 by Registry (Talk | contribs)

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J23119-TCI42-pL(3)-pR(3)-mRFP

This part contains the coding gene of the thermo-sensitive transcription factor TCI42 under the constitutive promoter J23119 and a reporter gene RFP under the promoter of TCI42. When the temperature is below 35℃, TCI42 is active to repress the transcription of RFP so that there will be little fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1658
    Illegal AgeI site found at 1770
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None