Coding

Part:BBa_K2984027

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 10:04, 16 October 2019 by Registry (Talk | contribs)

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L1c-Psad-ARS-YFP-Rbcs2

L1c plasmid containg arylsulfatase secretion signal and mVEnus fluorescent marker which are flanked by Psad Promoter and Rbcs2 Terminator. The construct is designed to test for the efficency of the secretion signal voa fluorescence intensity measurements.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1110
    Illegal NgoMIV site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisC.reinhardtii
emission528 nm
excitation515 nm