Composite

Part:BBa_K3239010:Design

Designed by: Xingyu Zhou   Group: iGEM19_ShanghaiFLS_China   (2019-10-16)
Revision as of 09:56, 16 October 2019 by BrianSebZhou (Talk | contribs) (→‎Source)


pPRM1-MIT1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2995
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2995
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 293
    Illegal BglII site found at 3453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2995
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2995
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Parts were fused via Gibson assembly, hence there were no scars.


Source

P.pastoris GS115 and https://www.ncbi.nlm.nih.gov/nuccore/82569479

Registered constructs BBa_K3239000, BBa_K3239004

References