Composite

Part:BBa_K3239011:Design

Designed by: Xingyu Zhou   Group: iGEM19_ShanghaiFLS_China   (2019-10-16)
Revision as of 09:54, 16 October 2019 by Registry (Talk | contribs)

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pMIT1-PRM1-pPRM1-MIT1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2034
    Illegal NheI site found at 3815
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2034
    Illegal BglII site found at 1387
    Illegal BglII site found at 4268
    Illegal BglII site found at 7428
    Illegal BamHI site found at 2917
    Illegal BamHI site found at 3836
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The two subparts shall be separately homologously integrated into the pAOX1-GFP strain.


Source

P.pastoris GS115 BBa_K3239010 and BBa_K3239009

References