Composite

Part:BBa_K3102035:Design

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 09:54, 16 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎Design Notes)


T7-RBS-DLDII Operon-Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4365
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the DLD-II operon, we mutated a Cysteine into Thymine and a Guanine into Adenine at the 428bp and 433bp position (C428T & G433A), respectively, because of the PstI enzyme. We also mutated a Adenine into Guanine and a Cysteine into Thymine at the 2799bp and 2802bp position (A2799G & C2802T), respectively, because of the EcoRI enzyme.

Source

This sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1405-MONOMER#tab=TU

Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Kouzuma A, Kasai T, Hirose A, Watanabe K. "Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells". Front Microbiol. 2015;6(JUN):1–11.