Part:BBa_K3089011

T7 promoter+csgA-linker-mfp5-His fusion protein

This composite part is meant to express csgA-linker-mfp5 fusion genes under T7 promoter and purify CsgA-linker-Mfp5 recombinant proteins by His-tag affinity purification. CsgA is an amyloid-like protein encoded on the genome of E.coli MG1655 providing mechanical cohesive strength and Mfp5(BBa_K1583002)is a mussel foot protein from Mytilus galloprovincialis responsible for interface adhesion. This recombinant protein would self-assemble into fibrous bundles or films with adhesiveproperties by displaying the mussel adhesion domains on the surface of amyloid scaffolds, which would be a promising new generation of bio-inspired adhesives for a wide range of applications. This part was designed based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres" (Zhong et al, 2014.)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 76
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]