Composite

Part:BBa_K3102045:Design

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-15)
Revision as of 09:07, 16 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎Design Notes)


Synthesis and externalization of lactate (DLD-II Operon, MtrC)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4949


Design Notes

We mutated the MtrC sequence with a Guanine into Cysteine and a Guanine into Adenine at the 1838bp and 1841bp position (G1838C & G1841A), respectively, because of the PstI enzyme (BBa_K3102020).

Source

DLD-II: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1405-MONOMER#tab=TU

MtrC: sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1627

Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Kouzuma A, Kasai T, Hirose A, Watanabe K. "Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells". Front Microbiol. 2015;6(JUN):1–11.