Composite

Part:BBa_K3102046:Design

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-15)
Revision as of 08:24, 16 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎Source)


Electrons production (pflB, ACS, FDH, MDH)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6180
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5516
    Illegal AgeI site found at 534
    Illegal AgeI site found at 3413
    Illegal AgeI site found at 6024
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 836


Design Notes

The ACS sequence is mutated a Cytosine into Guanine at the 1609bp position (C1609T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. (e.g. BBa_K3102022).

The MDH sequence is mutated an Adenine into Guanine at the 829bp position (A829G) because of the EcoRI enzyme. (e.g. BBa_K3102018)

Source

PFLB: sequence comes from Shewanella Oneidensis and was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-2686

ACS: sequence comes from Shewanella Oneidensis and was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-2522#tab=TU

FDH: sequence comes from Saccharomyces cerevisiae was found through Yeastgenome: https://www.yeastgenome.org/locus/S000005915

MDH: sequence comes from Shewanella Oneidensis and was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-721

Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References