Composite
Part:BBa_K3102046:Design
Designed by: Chloe DOIZELET Group: iGEM19_Ionis_Paris (2019-10-15)
Electrons production (pflB, ACS, FDH, MDH)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6180
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 5516
Illegal AgeI site found at 534
Illegal AgeI site found at 3413
Illegal AgeI site found at 6024 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 836
Design Notes
The ACS sequence is mutated a Cytosine into Guanine at the 1609bp position (C1609T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. (e.g. BBa_K3102022).
The MDH sequence is mutated an Adenine into Guanine at the 829bp position (A829G) because of the EcoRI enzyme. (e.g. BBa_K3102018)
Source
none