Composite
phiC31TLU1

Part:BBa_K3254010

Designed by: Yeqing Zong, Li Haoyuan   Group: iGEM19_GENAS_China   (2019-10-10)
Revision as of 08:15, 16 October 2019 by Zongyeqing (Talk | contribs)


Translational Unit for phiC31 integrase

This part can be placed downstreamed a prmoter. The thermodynamic characteristics of this part were described in the workframe of a previous study(see reference). The RiboJ(BBa_K2615996) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1764
    Illegal SapI.rc site found at 1455
    Illegal SapI.rc site found at 1513
    Illegal SapI.rc site found at 1618


[edit]
Categories
//function/recombination
Parameters
None