Composite

Part:BBa_K2927066

Designed by: KUO, PEI-CHING   Group: iGEM19_CCU_Taiwan   (2019-09-04)
Revision as of 07:16, 16 October 2019 by Harrisman (Talk | contribs)

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T7 promoter-guide RNA-crRNA 6-mutated crRNA 3

This set of DNA fragments is used to express normal and mutated crRNA through T7 promoter. The normal crRNA cooperates with Cas12a to detect specific DNA sequence in African swine fever virus (ASFV), while mutation abolish the interaction between crRNA and target sequence, which is served as negative control to confirm the specificity of crRNAs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None