Composite

Part:BBa_K3239010:Design

Designed by: Xingyu Zhou   Group: iGEM19_ShanghaiFLS_China   (2019-10-16)
Revision as of 07:14, 16 October 2019 by Registry (Talk | contribs)

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pPRM1-MIT1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2995
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2995
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 293
    Illegal BglII site found at 3453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2995
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2995
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Parts were fused via Gibson assembly, hence there were no scars.


Source

P.pastoris GS115 BBa_K3239000 BBa_K3239004

References