Coding
INT8

Part:BBa_K3254008:Design

Designed by: Yeqing Zong   Group: iGEM19_GENAS_China   (2019-10-14)
Revision as of 07:10, 16 October 2019 by Zongyeqing (Talk | contribs)


Integrase Int8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 930
    Illegal AgeI site found at 1327
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was first reported in 2014. In this version, a PstI site and an EcoRI site were eleminated by synonymous mutations.


Source

The original organism is the Staphylococcus haemolyticus JCSC1435. The DNA of this part was totally synthesised by commerical company.

References

Yang, L., et al., Permanent genetic memory with >1-byte capacity. Nat Methods, 2014. 11(12): p. 1261-6.