Coding

Part:BBa_K3058000

Designed by: Fung Chi Tuen   Group: iGEM19_Hong_Kong_LFC_PC   (2019-09-10)
Revision as of 06:50, 16 October 2019 by Tochingyuet (Talk | contribs)


Uricase from Cyberlindnera jadinii

This part encode a uricase (from Cyberlindnera jadinii) under the control of the constitutive promoter J23100 and strong RBS B0034. The uricase encoded can covert uric acid into hydroxyisourate. In our project, we express it in E. coli. It is hoped that the uricase-expressing E.coli can lower the uric acid concentration in human alimentary canal, so as to prevent gout disease.

T--Hong_Kong_LFC_PC--Uricasecolor2.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 510
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 532
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None