Intermediate

Part:BBa_I746911:Design

Designed by: Stefan Milde   Group: iGEM07_Cambridge   (2008-09-30)
Revision as of 09:40, 1 October 2008 by Stefan (Talk | contribs) (→‎Source)

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construction intermediate: T7 promoter - RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no special considerations


Source

The part was created from parts of the 2007 (B0034) and 2008 (I719005) part distributions. Due to the small size of both BioBricks involved a non standard assembly was carried out, BUT the result is exactly (!) the same as if standard assembly had been carried out:

Insteady of cutting with EcoRI + SpeI (for the promoter) and EcoRI + XbaI (for the RBS) the parts were cut with PvuI + SpeI (for the promoter) and PvuI + XbaI (for the RBS).

The PvuI site is located in the amp resistance gene of the pSB1A2 vector which both parts were located in. Hence larger fragments could be used in the standard assembly.

The resulting sequence, however, is identical to what would have been achieved by standard assembly with the four standard restriction enzymes.

References