Composite

Part:BBa_K3001003

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-16)
Revision as of 05:32, 16 October 2019 by Registry (Talk | contribs)

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Cas13a Lbu + T7 promoter

Inducible construct for Cas13a Lbu with purification tags.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 107
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 297
    Illegal BglII site found at 845
    Illegal BglII site found at 1781
    Illegal BglII site found at 1991
    Illegal BglII site found at 2045
    Illegal BglII site found at 2276
    Illegal BglII site found at 2549
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
    Illegal NgoMIV site found at 2497
    Illegal NgoMIV site found at 3133
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None