Composite

Part:BBa_K3239009:Design

Designed by: Xingyu Zhou   Group: iGEM19_ShanghaiFLS_China   (2019-10-15)
Revision as of 04:19, 16 October 2019 by Registry (Talk | contribs)

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pMIT1-PRM1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2034
    Illegal NheI site found at 3815
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2034
    Illegal BglII site found at 1387
    Illegal BamHI site found at 2917
    Illegal BamHI site found at 3836
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the parts were fused together via Gibson assembly, hence there's no scar.


Source

P. pastoris GS115

References