Coding
Part:BBa_K3031018
Designed by: David Doyle Group: iGEM19_SUIS_Shanghai (2019-10-15)
OxyR mutated at residues 147 & 203
OxyR Transcription factor protein. OxyR reacts with H2O2 resulting in the oxidation of a reactive cysteine (Cys-199) formation of a intermolecular disulfide bond and thus a conformation change in the OxyR protein. The activated OxyR then binds to certain promoter regions to regulate the expression of downstream genes.
This is an improvement to the part BBa_K1104200 submitted by National Yang Ming University iGEM Team 2013.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 720
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 673
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Categories
Parameters
None |