Generator
P43-spoVG

Part:BBa_K143051

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-09-30)
Revision as of 11:53, 30 September 2008 by Registry (Talk | contribs)

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Promoter 43 and RBS spoVG for B. subtilis

Constitutive promoter 43(BBa_K143013) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis.

P43-spoVG can be used in the context of a Polymerase per second (PoPS) output generator.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/Bsubtilis
//chassis/prokaryote/mflorum
Parameters
None