Part:BBa_K3317051:Design

NhaS with an RFP reporter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Unknown
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The promoter was modified for its constitutive production and its characterization, adding the BBa_J23119 part at the beginning of the sequence and replacing its UV-activated promoter from the original BBa_K1255002 part.

Source

The original sequence was described by Krulwich in 1994 as a hypothetical gene. However, this part has been optimized from the BBa_K1255002 part registered by the CIDEB-UANL_Mexico team in 2014.

References

CIDEB-UANL_Mexico. (2014). Capture module. Recuperado el 24 de abril del 2018 de http://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture. Huang, L. (2010). Growth kinetics of Escherichia coli O157:H7 in mechanically-tenderized beef. International Journal of Food Microbiology. No. 140, pp. 40–48. Krulwich, T.& Ivey, M. (1994) Sodium ion binding proteins. Recuperado el 24 de abril del 2018, de http://www.google.com.mx/patents/US5346815.