Device

Part:BBa_K3317051:Design

Designed by: Ral Acosta Murillo   Group: iGEM19_UANL   (2019-10-15)
Revision as of 22:53, 15 October 2019 by RollerCoaster (Talk | contribs) (→‎References)


NhaS with an RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 849
    Illegal AgeI site found at 961
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter was modified for its constitutive production and its characterization, adding the No part name specified with partinfo tag. part at the beginning of the sequence and replacing its UV-activated promoter from the original No part name specified with partinfo tag. part.


Source

The original sequence was described by Krulwich in 1994 as a hypothetical gene. However, this part has been optimized from the No part name specified with partinfo tag. part registered by the CIDEB-UANL_Mexico team in 2014.

References

CIDEB-UANL_Mexico. (2014). Capture module. Recuperado el 24 de abril del 2018 de http://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture. Huang, L. (2010). Growth kinetics of Escherichia coli O157:H7 in mechanically-tenderized beef. International Journal of Food Microbiology. No. 140, pp. 40–48. Krulwich, T.& Ivey, M. (1994) Sodium ion binding proteins. Recuperado el 24 de abril del 2018, de http://www.google.com.mx/patents/US5346815.