DNA

Part:BBa_K2940003

Designed by: Francisco Ivan Rodriguez Jaubert   Group: iGEM19_Edinburgh_OG   (2019-10-15)
Revision as of 21:36, 15 October 2019 by Registry (Talk | contribs)

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Synthetic silk MaSp1 with flanking solubilizing blocks and head-to-tail assembly system

Ready to use Golden Gate multimerization assembly to create synthetic silk MaSp1 monomer repeats. Flanking the MaSp1 gene, five glutamic acids had been added as solubilizing blocks. The composite presents orthogonality since the MaSp1 can be changed for any other part desired to create monomer repeats.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 128
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
    Illegal SpeI site found at 128
    Illegal NotI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 128
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 128
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None