Coding

Part:BBa_K3084000

Designed by: Lucas Coppens   Group: iGEM19_KU_LEUVEN   (2019-10-15)
Revision as of 19:25, 15 October 2019 by Registry (Talk | contribs)

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Venus Yellow Fluorescent Protein (YFP), S-TIP37 codon optimised

Our team wanted to engineer the genome of an S-TIP37 phage to express a protein upon infection. In order to have the best expression possible, we looked into the proteins in the phage genome that are strongly expressed. Since translational efficiency is a strong determinator of the scale of expression, yellow fluorescent protein was codon-optimized for S-TIP37 phage capsid protein, in order to obtain a strong expression upon infection of Synecoccocus WH8109

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 424
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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