Coding
Part:BBa_K3084000
Designed by: Lucas Coppens Group: iGEM19_KU_LEUVEN (2019-10-15)
Venus Yellow Fluorescent Protein (YFP), S-TIP37 codon optimised
Our team wanted to engineer the genome of an S-TIP37 phage to express a protein upon infection. In order to have the best expression possible, we looked into the proteins in the phage genome that are strongly expressed. Since translational efficiency is a strong determinator of the scale of expression, yellow fluorescent protein was codon-optimized for S-TIP37 phage capsid protein, in order to obtain a strong expression upon infection of Synecoccocus WH8109
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 424
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 682
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |