Composite

Part:BBa_K3102043

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-15)
Revision as of 18:22, 15 October 2019 by Registry (Talk | contribs)

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Lactate production (ldhA, lldP, ACS operon)

This lactate production (LDHA, LldP, ACS operon) composite part composes of Lactate dehydrogenase (LdhA):BBa_K3102026 and lactate permease (LldP):BBa_K3102027 sequences increase the production and secretion of D-Lactate in the media. The LDHA enzyme catalyses the interconversion of pyruvate and L-lactate with inter-convertion of NADH and NAD+.

This composites also constitutes of the ACS operon:BBa_K3102028, composed of three genes actP (Transports acetate) acs (catalyzes the conversion of acetate into acetyl-CoA) and yfcH (epimerase) uptake acetate molecules in order to allow the bacteria to do its metabolism and consequently survive.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1478
    Illegal XhoI site found at 3404
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 489
    Illegal AgeI site found at 1614
    Illegal AgeI site found at 2077
    Illegal AgeI site found at 2515
    Illegal AgeI site found at 2674
    Illegal AgeI site found at 2686
    Illegal AgeI site found at 3833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 219
    Illegal SapI.rc site found at 6698


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Categories
Parameters
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