DNA

Part:BBa_K3139001:Design

Designed by: Jiapei Han   Group: iGEM19_NAU-CHINA   (2019-10-15)
Revision as of 18:12, 15 October 2019 by Registry (Talk | contribs)

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A/U-rich 5-UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The length and A/U content of an A/U-rich 5'-UTR should be various from species, different hosts should have the most suitable one. Our design is to be used in E.coli DH5α. For further application, different mutation should be applied and tested.


Source

E.coli hly operon, mutated from the upstream of the hlyA gene.

References