Composite

Part:BBa_K2912015

Designed by: Lingling Liao   Group: iGEM19_SZU-China   (2019-10-13)
Revision as of 17:59, 15 October 2019 by EmrysTang (Talk | contribs) (→‎Usage and Biology)


Trp_Lysis gene

Trp_Lysis gene expression can be controlled by the concentration of Tryptophan

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The lysis gene from colicin-producing strains of bacteria encodes the lysis protein which can cause the host cell to lyze as long as the protein is activated. On the basic of its biological function, we can use lysis gene to break our engineering E.coli and release the interior cell contents when it's time to extract our product-shRNA by adding a Tryptophan attenuator between a RBS and lysis gene

We transformed the expression vector into HT115 (DE3) E.coli by heat-shock method



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