Composite

Part:BBa_K2996709

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-15)
Revision as of 17:01, 15 October 2019 by Pedestal (Talk | contribs)


LuxI downstream of activation unit containing lure

LuxI is put downstream of BBa_K2996705, as a reporter, which is a synthetase for acyl-homoserine lactones (AHL). dcas9 binding to lure means the occurrence of off-target incidence. RpoA, which is linked to dcas9, will recruits RNA polymerase and the gene downstream of the promotor will be activated. We develop a way to record off-target incidence, with LuxI gene under the control of promotor J23117. Increased expression of luxI enzyme will synthesis enough AHL molecules, which can penetrate to the bacteria capable of information storage.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
    Illegal NheI site found at 75
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 743
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 39
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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