Composite

Part:BBa_K3102042:Design

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-15)
Revision as of 16:43, 15 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎References)

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Cellulose Acetate degradation (CAE, EG1, Tev, HlyA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2958
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 124
    Illegal NgoMIV site found at 1234
    Illegal NgoMIV site found at 4120
    Illegal NgoMIV site found at 4426
    Illegal AgeI site found at 254
    Illegal AgeI site found at 341
    Illegal AgeI site found at 479
    Illegal AgeI site found at 662
    Illegal AgeI site found at 710
    Illegal AgeI site found at 878
    Illegal AgeI site found at 1091
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

CAE, Neisseria Sicca, sequence found through genebank : https://www.ncbi.nlm.nih.gov/protein/BAJ21448.1

EG1, Neisseria Sicca, sequence found through genebank https://www.ncbi.nlm.nih.gov/protein/BAJ21449.1

TEV protease, Tobacco etch virus, sequence found through Uniprot https://www.uniprot.org/uniprot/Q0GDU8


Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Moriyoshi K, Ohmoto T, Ohe T, Sakai K. Role of Endo-1,4-β-glucanases from Neisseria sicca SB in synergistic degradation of cellulose acetate. Biosci Biotechnol Biochem. 2003;67(2):250–7.

Phan, J., Zdanov, A., Evdokimov, A. G., Tropea, J. E., Peters, H. P. K., Kapust, R. B., Li, M., Wlodawer, A., and Waugh, D. S. (2002). Structural basis for the substrate specificity of tobacco etch virus protease. J. Biol. Chem. 277: 50564-50572.