Coding
YFP B3-B4

Part:BBa_K2984017:Design

Designed by: Sophia Schiebler   Group: iGEM19_Humboldt_Berlin   (2019-10-14)
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YFP - Yellow fluorescent protein for detection B3-B4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 220
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 220
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This was designed as a B3-B4 part according to the MoClo standard of Patron et al. (2015).


Source

Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., & Remington, S. J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science, 273(5280), 1392-1395.

References

Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., & Remington, S. J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science, 273(5280), 1392-1395. Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., . . . Haseloff, J. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. Retrieved from https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.13532.