Part:BBa_K3279007
β-Glucosidase from Streptomyces coelicolor
β-Glucosidase obtained from various sources has been used in many areas, such as the enzymatic saccharification of cellulosic materials, the liberation of flavor compounds in fruit juices and wines, and so on. In cellulose hydrolysis, β-glucosidase is used to degrade cello-oligosaccharides to glucose[1]. We used this part to accomplish the cellulose degradation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 287
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 427
Illegal NgoMIV site found at 1103
Illegal AgeI site found at 184 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 687
Illegal BsaI.rc site found at 946
Characterization from iGEM19_CAU_China
We linked β-glucosidase coding sequence into a pET30a(+) backbone, then transformed this plasmid into BL21. After the overnight (about 15h) induction under the condition of 16℃ 0.08 mM（see Fig.1[1]）, we smashed the recombinant E.coli strain with the ultrasonication to get crude enzyme solution, then we measured the enzyme activity by the method of CMC-Na(sodium carboxymethyl cellulose) assay. Considering the relatively weak cellulose degradation capacity of β-glucosidase, we mixed the crude enzyme solution with endoglucanase. The result is shown on Fig.2[1].
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