Plasmid

Part:BBa_K3253005

Designed by: HAOYU JIANG   Group: iGEM19_Nanjing   (2019-10-15)
Revision as of 15:45, 15 October 2019 by Registry (Talk | contribs)

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pCAMBIA2301

pMD-18T is the cloning vector we use to clone our gene of interest.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 10821
    Illegal XbaI site found at 10848
    Illegal PstI site found at 10860
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 10821
    Illegal NheI site found at 2025
    Illegal NheI site found at 5470
    Illegal PstI site found at 10860
    Illegal NotI site found at 3344
    Illegal NotI site found at 4877
    Illegal NotI site found at 6167
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 10821
    Illegal BglII site found at 7
    Illegal BglII site found at 9778
    Illegal BamHI site found at 10842
    Illegal XhoI site found at 8913
    Illegal XhoI site found at 9791
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 10821
    Illegal XbaI site found at 10848
    Illegal PstI site found at 10860
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 10821
    Illegal XbaI site found at 10848
    Illegal PstI site found at 10860
    Illegal NgoMIV site found at 2789
    Illegal NgoMIV site found at 2963
    Illegal NgoMIV site found at 3013
    Illegal NgoMIV site found at 3017
    Illegal NgoMIV site found at 3044
    Illegal NgoMIV site found at 3543
    Illegal NgoMIV site found at 3901
    Illegal AgeI site found at 5964
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5867
    Illegal SapI site found at 6558
    Illegal SapI site found at 9070
    Illegal SapI site found at 9280
    Illegal SapI.rc site found at 6095


[edit]
Categories
//plasmid/construction
Parameters
functionThis is a cloning vector which has several restriction enzyme binding site.