Part:BBa_J52008

luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)

Rluc is a protein called Renilla luciferase and it emits light when the right substrate is added. That is why it can be used as a reporter to track other proteins or to monitor the activity of a promoter.

Usage and Biology

This part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix. See the sequence first.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 547
  

experiment

We transferred the plasmid with only carrying hRluc and stimulated them with different concentration of sugar. With the purpose of detecting the correlation between the expression of hRluc and the stimulation of the sugar’s concentration, we tracked the cells and used the double luciferase kit to test their correlation. And the result showed below.

https://2019.igem.org/wiki/images/b/be/T--NUDT_CHINA--result_of_SV40_promoter.png%7C130px%7C