Coding

Part:BBa_K3279008:Design

Designed by: Liang Siwen   Group: iGEM19_CAU_China   (2019-10-15)
Revision as of 15:22, 15 October 2019 by SvenL (Talk | contribs) (→‎Source)


cenA gene fused with INP-N sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 333
    Illegal BamHI site found at 733
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
    Illegal NgoMIV site found at 408
    Illegal AgeI site found at 1051
    Illegal AgeI site found at 1414
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primary concern was BioBrick compatibility so that illegal sites in RFC[10] did not appear in the sequence. And the sequeces was finally designed to link with pET30a(+) , so we added restriction enzyme cutting site NdeI at the 5' and SalI at the 3'.


Source

cenA gene (BBa_K118023) is from Cellulomonas fimi genomic DNA. INP-N sequence (BBa_K3279006) is originally from Pseudomonas syringae genomic DNA and we (CAU_China 2019) did a codon optimization.

References