Part:BBa_K3096048

N-Acetyl-Glucosamin-6 phosphate regulated repression system

This is a composite part designed by iGEM Tübingen for the N-Acetyl Glucosamine dependent expression of a Mnt repressor.

Usage and Biology

Commensal bacteria in the gut metabolize the mucus within the intestines, which increases the level of GlcNAc within the lumen. GlcNAc is taken up by the bacteria through their PTS system and metabolized into GlcNAc-6-P, which binds the repressor protein nagC. If nagC is bound to GlcNAc-6-P it loses ist abilities to bind DNA, and therefore ist respective regulation activity. In our case, nagC can consequently not serve as a repressor of the nagBACDE, anymore. Thus, a repressor Protein, the Mnt repressor of the Lambda Phage, can be expressed. The Mnt repressor binds to the Mnt promoter inhibiting the transcription of downstream genes. If GlcNAc-6-P is not present nagC remains active repressing the Expression of the MntR.

Sequence and Features

The NagC generator produces a constitutively expressed NagC which - in the absence of GlcNAc6P - inhibits pNagC. pNagC determines the expression of MntR.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 572
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Sicard JF, Le Bihan G, Vogeleer P, Jacques M, Harel J. Interactions of Intestinal Bacteria with Components of the Intestinal Mucus. Front Cell Infect Microbiol. (2017); 7:387. Published 2017 Sep 5. doi:10.3389/fcimb.2017.00387
2. Barnhart MM, Lynem J, Chapman MR. GlcNAc-6P levels modulate the expression of Curli fibers by Escherichia coli. J Bacteriol. (2006); 188(14):5212–5219. doi:10.1128/JB.00234-06
3. Konopka JB. N-acetylglucosamine (GlcNAc) functions in cell signaling. Scientifica (Cairo). (2012); 2012:489208. doi:10.6064/2012/489208