Coding

Part:BBa_K3241000:Design

Designed by: Ruxiang Wang   Group: iGEM19_HUBU-WUHAN   (2019-10-15)
Revision as of 15:10, 15 October 2019 by ZERO-Wang (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


PhbA(Beta-ketothiolase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1126
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 310
    Illegal NgoMIV site found at 715
    Illegal NgoMIV site found at 844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 141


Design Notes

Source

This gene is originally from Ralstonia eutropha.GenScript synthesized the gene and optimized its codon for us. This gene was cloned in pUC57.

References